In the present study, the hepatocytes were obtained through shearing 9-day-old Sanhuang chicken embryo liver tissue, separating by trypsin digestion and culturing in revised M199 culture.

• 摘要剪切9日龄三黄肉鸡鸡胚肝组织，胰酶消化分离肝细胞，用改良的M199培养液进行培养。