In this experiment, differential expressing genes in leg muscle of chicken mentioned above were studied via DDRT-PCR technique, which firstly adopted 72 primer pairs that piloted by 3 T7 (dT11) N as anchor primers and 24 M13-ARPs as random primers.

• 本实验采用3个26bp的T7(dT11)N为锚定引物,24个26bp的M13-ARPs为随机引物,组成72个引物组合对上述四个品种雏鸡腿肌组织进行DDRT-PCR反应。