In this research,the gene encoding phospholipase D from Streptomyces chromofuscus was cloned into expressed vector pET-22b(+) constituting recombinant plasmid pET-pld which was tranformed into E. coli BL21(DE3).

• 利用表达载体pET-22b(+),实现了色褐链霉菌磷脂酶D基因在大肠杆菌BL21(DE3)中的高效表达。