In this study the SerB gene was obtained from E. coli Jm109 by PCR and inserted into pEC7 and this recombinant plasmids with SerB gene were transformed into Brevibacterium flavum C-l1 (BfC-11 ) by the method of electrotransformation.

• 本研究应用PCR技术从大肠杆菌JM109(E.；coli JM109)中扩增出磷酸丝氨酸转氨酶基因，将其与表达载体pEC 7连接。