In this study,the mammalian condon optimized F gene was synthesized by over-lapping PCR and used to generate recombinant vaccinia virus,rWR-NiV-F.

• 通过over-lapping PCR合成密码子优化的F蛋白基因构建了表达NiV F蛋白的重组牛痘病毒(WR株)rWR-NiV-F。