In this thesis, Agrobacterium tumefaciens mediated transformation (ATMT) approach was used to screen insertional mutagenesis and the factors that affected efficiency of transformation were optimized.

• 本研究通过一系列农杆菌介导的转化(Agrobacterium tumefaciens mediated transformation, ATMT)试验，摸清了影响转化效率的主要因素；