In this thesis, ribonuclease A (RNase A) was employed as a model protein to understand the formation of correct three-dimensional structure in the refolding of recombinant proteins in vitro. On this basis, the refolding mechanism in vitro was discussed.

• 本文以核糖核酸酶A(RNase A)为蛋白质体外重折叠的模型蛋白,对其体外重折叠过程进行了研究,探讨了这类目标蛋白复性过程的一些规律性的问题。