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- Conclusions It indicated that the method of in situ RT PCR is specific and sensitive,and will be useful for research on coxsackie virus B sustainable infection and related diseases. 而非感染的HeLa细胞则不着色。 结论原位RT－PCR法可特异地检测柯萨奇B病毒感染HeLa细胞中的病毒RNA，是一种特异和敏感的定位检测方法，可应用于研究该病毒的持续性感染及其与克山病等相关疾病的关系
- Experimental study on detection of viral RNA of Coxsackie virus B by means of in situ RT PCR 原位RT-PCR法检测柯萨奇B病毒RNA的实验研究
- Methods RT polymerase chain reaction(RT PCR), immunohistochemical methods and in situ hybridization technologies were used to detect the PCNA, L faabp mRNA level and located distribution at embryonic period and natal period. 方法利用免疫组织化学、原位杂交和反转录聚合酶链反应(RT-PCR)等技术,分别检测胚胎期E14d,E17d,E19d和幼鼠期P1d,P2d,P7d,P28d大鼠小肠上皮细胞增殖细胞核抗原(PCNA)、L-fabpmRN水平及其定位分布。
- RT PCR was performed for APN gene expression in liver tissue. (2 )APN表达 :根据兔APN基因cDNA序列设计引物 ,提取肝总RNA ,利用RT PCR方法 ,检测肝APNmRNA水平的变化。
- in situ RT - PCR 原位RT-PCR
- y means of RT PCR and in situ hybridization with digoxigenin labeled IL 6 cDNA probe,we detected the expression of IL 6 mRNA in kidneys of patients with lupus nephritis and normal persons as control. 为探讨IL－6在狼疮性肾炎病人肾脏组织中的表达情况及其致病作用，我们用RT－PCR和原位杂交方法对狼疮性肾炎病人和正常人肾穿标本中的IL－6mRNA进行检测。
- Methods Plasma ETA was measured quantitively by RT PCR. 方法 应用 RT-PCR技术检测外周血循环中 ETA基因表达水平。
- Key around Mobile, the next key stop in situ fixed. 左右键移动,下键停在原地不动。
- Lobular carcinoma in situ is seen here. 图示小叶原位癌。
- Fluorescence in situ hybridization See FISH. 荧光原位杂交：参见FISH.
- RDP＝rumen-degradable protein measured in situ. 经瘤胃消化袋测得之瘤胃可分解蛋白质。
- Angiotensin receptor (ATR) mRNA expression of VSMC was measured by RT PCR. RT PCR检测VSMC血管紧张素受体 (ATR)mRNA表达。
- Biopolymer can be triggered in situ to form a gel. 对生物矿化作用的进一步研究有助于微生物调剖机理的完善和应用。
- Expression of the M 2-1 gene was examined by RT PCR and Western blot. 重组真核质粒PXJ 4 1/M2 -1转染肺腺癌PAa细胞 ,应用RT PCR、Westernblot方法检测表达。
- The expression of cyclin D1 and CDK4 were detected by RT PCR method. RT PCR检测细胞周期素D1(cyclinD1)、细胞周期蛋白依赖激酶 4 (CDK4 )mR NA的表达。
- The rocks were not in situ; they had been brought from elsewhere. 石块不是原来就在那里的，是从别处搬来的。
- In situ deposits are dark shale with horizontal bedding. 原地沉积为水平层理发育的暗色泥板岩。
- Methods:Nine Chinese AIDS kidney autopsies were detected by PCR in situ . 方法:应用原位PCR方法检测9例国人艾滋病尸体解剖肾组织标本。
- RT PCR for EWS FLI 1 is sensitive enough to detect minimal residual Ewing's sarcoma cells in the bone marrow. 以EWS FLI 1融合基因为标志的反向PCR测定骨髓Ewing肉瘤细胞的灵敏度为 10 -6,它是测定骨髓Ewing肉瘤细胞高度敏感的方法 ;
- In Situ Hybridization on Vasa Gene of Silkworm Bombyx Mori. 家蚕vasa基因的原位杂交研究
