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- Keywords hepatocarcinoma targeting therapy;in vivo phage display technology;specifically binding peptide;tissue chips;immunofluorescence assay;magnetic nano particles;immunotoxin;cell-based ELISA; 肝癌靶向治疗;体内噬菌体展示技术;特异性粘附肽;组织芯片;免疫荧光技术;纳米磁性材料;免疫毒素;细胞ELISA;
- Research Progress of Screening in vivo of Phage Display 噬菌体展示技术体内筛选的研究进展
- Keywords Phage display techniques In vivo Biopanning Targeted therapy; 噬菌体展示技术;体内筛选;靶向治疗;
- Phage display in vivo 噬菌体体内展示
- Adl2 ElB 55kDa binds hDaxx in vivo or in vitro. 共免疫沉淀反应和Western blot结果证实Ad12 E1B 55KD能在细胞内外与hDaxx直接结合。
- The expression product shows activity of antitumor in vivo. 表达产物在体内具有一定的抗肿瘤活性。
- Result Nicardipine can induce rat mesangial cell apoptosis in vivo. 结论尼卡地平在体内有诱导大鼠系膜细胞凋亡的作用。
- Methods Using the in vivo micropuncture technique in SD r ats. 方法利用活体微穿刺技术。
- CONCLUSION Survivin T34A might inhibit A549 tumors growing in vivo. 结论 Survivin T34A在体内可明显抑制A549肺癌生长。
- In recent years, phage display was considered for vaccine development. 近年来,呈现噬菌体开始应用于疫苗的研究。
- At last, tumorigenesis assay was undertaken in nude mice in vivo. 进行黑素瘤细胞体外克 隆形成实验观察,计算各组细胞的克隆形成率;
- The embryos from the in vivo fertilization develop best. 体内受精胚胎的体外发育最佳。
- T1 phage displayed a higher survival than poliovirus. T1噬菌体的存活高于脊髓灰质炎病毒。
- And, these monkeys were injected in vivo dose must SIV virus. 然后,这些猴子体内均被注入一定剂量的SIV病毒。
- Conclusion The VH containing extra-sequence may exist in vivo. 结论提示这段异常序列在体内可能存在于该VH之中。
- Hie resultant vector was checked by expression of phage antibody, ELBA and in vivo recombination. 通过噬菌体抗体的表达、ELISA及细胞内重组试验鉴定所获得的表达载体。
- Here, the basic mechanism and the application of phage display random peptide libraries are briefly introduced. 本文简介此类文库的基本作用原理及应用。
- When phage in vivo circulated at enough long period of time, some phage particles could extravasate in some organs or tissues and internalized there. 当噬菌体在体内循环足够长的时间后;一些噬菌体颗粒可以穿透血管进入并内化在器官或组织中.
- Objective To screen polypeptides binding to human CD137 by biopanning phage display random heptapeptide library. 目的应用噬菌体随机七肽库筛选与人CD137特异结合的肽序列。
- Aim The phage display antibody library was constructed to obtain functional anti Fas Fab. 目的构建噬菌体抗体库,获得具有功能的抗Fas?Fab噬菌体抗体。