Isolation of adult DUM neurons was performed under sterile conditions using enzymatic digestion and mechanical dissociation of the median parts of the TAG from the nerve cord of the cockroach Periplaneta Americana.

• 使用酶消化和机械分离出成年美洲蜚蠊的腹部末端神经节(TAG)，然后在无菌条件下分离DUM神经元细胞。