It was proved that the E2 genes were integrated stably into chromosome of P.Pastoris by Dot blot and DNA sequencing.

• Pastotis进行整合，经G418筛选得到25个高拷贝转化子，经DNA斑点试验和DNA测序证明外源基因E2稳定地整合到P.；Pastoris染色体中。