It was testified that CP gene in pcDNA3.1-orf2, pcDNA4-orf2 or pIRES-orf2/gm-csf, GM-CSF gene in pcDNA3.1-gm-csf and pIRES-orf2/gm-csf could be expressed in COS7 cells by indirect immunofluorescene assay and colony-stimulation forming assay.

• 采用间接免疫荧光试验和集落刺激形成试验证明；在COS7细胞中pcDNA3.；1-gm-csf可表达GM-CSF；pcDNA3