METHOD VSMC were labelled with fluo 3 AM, a calcium sensitive fluorescent dye, and [Ca 2+ ] i was determined by the use of the adherent cells analysis and sorting apparatus(ACAS 570).
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- 方法 用荧光染料Fluo 3 AM标记细胞 ,在粘附式细胞仪上测定细胞内游离钙的变化。