METHOD: We extracted the RNA of Toxoplasma gondii (RH strain)by using a single step acid guanidinium thiocyanate method and isolated the poly(A) +RNA by using messenger RNA affinity membrane containing poly U to synthesize ds cDNAs.

• 方法：用异硫氰酸胍酸性一步法提取弓形虫（RH株）RNA，含PolyU的mRNA亲和膜分离Poly（A）＋RNA，进而合成双链cDNA。