METHODS: By establishing the C6 rat glioma models, after cryotherapy.TUNEL and Flow cytometry were employed to inspect the number of apoptotic cells and apoptotic rate respectively.

• 方法：建立大鼠C6脑胶质瘤颅内接种模型，通过TUNEL法及流式细胞仪分别检测其冷冻后凋亡数目及凋亡率。