METHODS: DRV-YH plaque-purified third with solid culture medium in Muscovry duck embryo fibroblast (DEF) was grown with 199 sultion in semiconfluent monolayers of chicken embryo fibroblast (CEF) .

• 方法:国内番鸭分离株(DRV-YH)首先利用固体培养基在番鸭成纤维细胞(DEF)上克隆纯化3次,然后用199培养液在鸡成纤维细胞(CEF)上增殖,收获的细胞培养物经3次冻融;