METHODS: Local field stimulation was delivered to freshly isolated rat single cardiac myocytes to generate action-potential-induced Ca 2+ transients (ACT) for assessing intracellular Ca 2+ release.

• 方法 :局部场刺激作用于成年大鼠心肌细胞 ,促使后者产生动作电位 ,进而诱发胞内钙瞬变 (ACT) ,由 ACT可估测胞内钙释放。