METHODS: PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.

• 方法：从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。