METHODS:The calvarian bone of new born rats was digested and cultured.Then the cytokine,spleen cells and bone marrow cells were added to stimulate the monocytes to form osteoclasts.

• 方法:采用新生大鼠颅盖骨消化法培养成骨细胞,再加入细胞因子、脾细胞、骨髓细胞,刺激单核细胞向破骨细胞的分化。