MSTN-Q could not be purified with 50%Ni-NTA resin affinity chromatography, but the purity of MSTN-Q was very high when multiple ultrasonication and washing was carried out.

• 由于MSTN-Q蛋白不能用Ni-NTA柱进行纯化，对其进行多次超声处理、洗涤后可获得纯度相对高的蛋白。