Mater i a Is and Methods Eukaryotic expression vector of EGFR antisense RNA, pLXSN-AE5? was transfected into SMMC-7721 by using Stearylamine/DOPE (SA liposome).

• 材料与方法：应用十八酰基胺阳离子脂质体（SA阳离子脂质体）将EGFR反义RNA真核表达载体pLXSN-AE5’导入SMMC-7721细胞中，G418抗性筛选出稳定克隆细胞。