Metho ds:The EGFR expression in human trophoblast cells of different pregnancy phases was localized by immunohistochemical methods(ABC),and the related quantity of EGFR mRNA was analyzed by RT-PCR method.

• 方法:采用免疫组织化学方法测定不同孕期滋养层细胞EGFR的表达,应用逆转录-聚合酶链反应(RT-PCR)技术对EGFRmRNA进行分析。