Method E.sakazakii, was isolated from infant milk powder samples of 32 portions, and was identified by routine bacterial culture, ordinary PCR and real-time PCR respectively.

• 方法分别用常规培养鉴定方法、常规PCR方法和实时荧光PCR方法对32份奶粉样品进行阪崎肠杆菌分离鉴定。