Method Semiquantitive evaluation of MMP 1 and TIMP 1 mRNA were determined by RT PCR in liver tissue and fat storing cells (FSC) respectively.

  • 方法 分别从正常和CCl4 肝纤维化大鼠的肝组织及FSC中提取RNA,应用逆转录?聚合酶链反应(RT?PCR)方法检测MMP?2 与TIMP?1 mRNA 水平。
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