Method To activate spleen cells of mice with Con A in vitro and then observe the effect of the ethanol extract on the proliferation and activation of the cells by the method of MTT, CFSE and flow cytometric analysis.

• 方法:用ConA刺激小鼠脾细胞,分别用四甲基偶氮唑盐染色法(MTT法),流式细胞术等方法观察结血蒿醇提物对细胞增殖和活化的作用。