Methods APC:T:B cell reactive system was established in vitro. T cell proliferation was analyzed by using 3H TdR incorporation in the blockade of B7/CD28 with B7 1mAb and CsA. B cell antibody production was assayed by using ELISA.

• 方法 在体外建立抗原递呈细胞 T淋巴细胞 B淋巴细胞反应系统 ,利用环孢素A(CsA)与抗B7 1单克隆抗体联用阻断B7:CD2 8共刺激途径 ,采用3H TdR法检测T淋巴细胞增殖 ,酶联免疫吸附试验 (ELISA法 )测定B淋巴细胞分泌的抗体。