Methods All of the seven exons and intron exon boundaries of antithrombin gene were analysed by PCR and direct sequencing of amplified PCR products from the propositus.

• 方法用PCR法对先证者抗凝血酶基因的7个外显子及其侧翼内含子序列进行扩增,PCR产物纯化后直接测序,检测其基因突变。