Methods Amplify the gene encoding human rhinovirus 3C protease and clone into expression vector pBV220.Transform the constructed recombinant plasmid pBV220-3C to E. coli BL21(Gold) for expression.

• 方法通过RT-PCR方法获得人鼻病毒3C蛋白酶基因,克隆入表达载体pBV220,构建非融合表达载体pBV220-3C,转化大肠杆菌BL21(Gold)进行表达。