Methods Anaerobic environment was created by using palladiu catalysis for culture of anaerobic and aerobic bacteria in bile of 178 patients with the tract infection.

• 方法采用厌氧培养箱,用抽气换气靶粒催化耗氧法造成厌氧环境,对178例胆管疾病病人胆汁进行了厌氧菌与需氧菌分离培养。