Methods Both rapid quantitati ve bioassay for NGF(-like) activity by measurement of neurite extension in NGF -induced PC12 cells and a following MTT technique to monitor cells alive were u sed.

• 方法采用经NGF诱导的PC12细胞,以NGF为阳性对照,通过对分化细胞百分率的测定和存活细胞的MTT染色,建立模型并对样品进行筛选。