Methods CFSE staining combined with fluorescent antibody labeling and flow cytometry are used to detect the proliferation kinetics of lymphocytes and their subsets after stimulation of colonel stimulators in vitro.

• 方法 利用荧光染料CFSE作为细胞标记,结合荧光标记的抗体,通过流式细胞仪检测淋巴细胞及其亚群体外增殖情况。