Methods Dorsal root ganglions (DRG) from rat embryos were isolated under microscope and dissociated in trypsin.The dissociated neurons were treated with 5-fluorouracil for purification.

• 方法取胚胎大鼠背根神经节，通过胰蛋白酶消化、抗有丝分裂药物纯化等方法获得背根神经节神经元。