Methods ECE-1 antisense RNA expression vector with green fluorescent protein as a reporter was constructed and transfected into 16HBE with Fugene 6 TM .

• 方法 构建ECE -1反义RNA表达载体 ,用脂质体 (FuGENG 6)转染人气道上皮细胞株 (16HBE) ,G418筛选得到稳定表达反义ECE -1RNA的细胞株 (anti -16HBE)。