Methods GDC was used to treat rat hepatocytes cultured in vitro and the apoptotic cells were observed andanalyzed with light microscope, transmission electron microscope, TUNEL in situ hybridization- DNA agarose gel electro-phoresis and flow cytometry.

• 方法以GDC为处理因素，体外培养大鼠原代肝细胞，分别用光镜、电镜、TUNEL原位杂交，流式细胞术和DNA电泳技术观察和分析GDC诱导大鼠肝细胞凋亡的作用。