Methods Human gastric cancer cell lines AGS and BGC-823 were chosen as target cells and CD40 signalling was stimulated by soluble CD40 ligand (sCD40L). The proliferation of tumor cells was measured by MTT and the flow cytometry.
英
美
- 方法人胃癌细胞株AGS和BGC-823为研究对象;以可溶性CD40配体(sCD40L)激发胃癌细胞的CD40信号;采用四氮唑盐(MTT)、流式细胞仪技术检测激发CD40信号对胃癌细胞生长增殖的影响;基因表达谱芯片Affymetrix U133A 2.;0检测CD40信号激活前后胃癌细胞株AGS基因表达谱变化。