Methods Immunohistochemical staining of Podoplanin and CD34 were used to detect microlymphatic density (MLD) and microvascular density (MVD) in 227 colorectal cancers.

• 方法利用淋巴管和血管特异标记物Podoplanin和CD34行免疫组化EnVision法检测227例结直肠癌及正常黏膜,观察MLD、MVD分布特点,分析其与临床病理参数之间的关系。