Methods In this study, a liquid chromatography-time of flight mass spectrometric method was developed and applied to characterize the metabolites of PAC-1 after incubation with human liver microsomes.

• 方法 在一定的人肝微粒体浓度中加入药物，温孵后，采用液相色谱-飞行时间质谱法对孵化体系中的代谢产物进行分析。