Methods MTT assay was wed to detect the effects of heparin on growth of human breast cancer cell line MDA-MB-231,enzyme linked sorbent assay (ELSA) were employed to detect the expressions of Hyase,HA and CEA of 8 human breast cancer cell lines.

• 方法用MTT法测定肝素对人乳腺癌细胞株MDA-MB-231生长的影响; 酶联吸附法测定8株人乳腺癌细胞培养上清液中Hyase、HA和CEA的表达。