Methods Polymerase chain reaction (PCR) based microsatellite polymorphism analyses were performed to detect LOH on chromosome 13,fluorescence labeled primers and Perkin Elmer 377 DNA Sequencer were applied.

• 方法 应用聚合酶链反应 (PCR)方法 ,采用荧光标记的引物和 377型DNA序列自动分析仪 ,分析了 2 0例GBM 13号染色体上 14个微卫星多态性标记的LOH。