Methods Protocorm-like bodies and callus of DCWL subcultured for 30 days was used as the explants,N6 was used as the basic culture with phytohormone added,and fungal extracts as the elicitor.
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- 取继代培养30d的铁皮石斛类原球茎作接种材料,以N6为基本培养基,加入植物激素,并以真菌提取物作诱导子,对类原球茎和愈伤组织进行诱导培养。