Methods The mRNA levels of ET-1,ETA and ETB were determined using RT-PCR in placenta villous from 30 cases of NP and 60 cases of PIH.

• 方法采用逆转录聚合酶链反应(RT-PCR)检测60例妊娠高血压疾病和30例正常妊娠胎盘绒毛组织ET-1、ETA和ETB的mRNA表达,并设立GAPDH内对照对PCR产物作半定量分析。