Methods The prepared tissues were cultured until outgrowth of fibroblasts.When confluence was reached, the fibroblasts were subcultured with trypsinization at 1: 3 split ratio.

• 方法采用组织块贴壁培养法进行成纤维细胞培养，待细胞从组织块中长出并融合成致密单层后，用胰蛋白酶消化，以1:3传代。