Methods The rat osteoblasts were isolated and cultured by enzyme digestion, and edherent cell analysis andsorting cytometer (ACAS) was employed to assay the change of intracellular Ca2+ concentration of rat osteoblasts.

• 方法酶消化法体外分离培养胎儿鼠成骨细胞，应用ACAS检测成骨细胞内Ca2+脓度的变化。