Methods The target DNA fragments of BDV p40 gene was obtained by PCR amplification and inserted into pEGFP-N1 reporter vector. The constructed recombinant plasmid was confirmed by PCR and DNA sequencing.

• 方法通过PCR方法扩增获得博尔纳病病毒p40基因的完整序列,将此片段定向克隆到pEGFP-N1载体多克隆位点区,筛选重组阳性菌株,提取重组质粒,利用PCR方法和核酸序列测定验证重组质粒构建的正确性。