Methods The two microsatellite markers were amplified by polymerase chain reaction(PCR), followed by 10% non denatured polyacrylamide gel electrophresis (PAGE) and DNA sequencing to analyze the PCR products.
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- 方法用聚合酶链反应(PCR)扩增与PKD1连锁的两个微卫星,10%25非变性聚丙烯酰胺凝胶电泳结合DNA序列测定检测PCR产物。