Methods hMSCs were isolated and expanded in culture and the phenotypes(CD34,CD44 or CD45) of hMSCs were identified by fluorescent -activated cell sorting(FACS).
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- 方法:体外分离培养扩增hMSCs,进行流式细胞仪分析鉴定其免疫学表型CD34、CD44和CD45,以未加一抗只加二抗的hMSCs作为平行对照组;