Methods omentum obtained from albino male rats of the Sprague Dawley strain, PMC were isolated and subculture by enzymatic disaggregation. The antibody to cytokeratin was used for identified by immunofluorescence technique.
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- 方法大网膜取自雄性SD大鼠,胰蛋白酶-EDTA消化法用于PMC的原代培养及传代,细胞角蛋白抗体的间接免疫荧光染色用于PMC的鉴定;