Methods: 201 type 2 diabetics, 4 idiopathic type 1 diabetics and 100 control subjects were included in the study.Two exons of IPF-1(including the coding and flanking regions) were amplified using PCR.

  • 方法:选择2型糖尿病201例、特发性1型糖尿病4例及正常对照组100例作为研究对象,对IPF-1基因的两个外显子序列(包括编码区及侧翼区)进行PCR扩增,PCR产物经纯化后,经ABI 377 DNA 全自动测序仪对所有PCR产物直接测序。
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