Methods: IRE-EGFP-esp1 plasmid was transfected into lung cancer cell line A549 via FUGENE 6 liposome, ploidy, and cell cycle were studied by flow cytometry combined with fluorescent staining,and DNA index (DI) and SPF were calculated.

• 方法:将IRE-EGFP-esp1质粒用Fu-GENE6脂质体转染到肺腺癌细胞A549,经G418筛选获得转染阳性的细胞系(esp1-A549)。 采用DNA荧光染色流式细胞仪分析转染后细胞DNA含量、倍性及细胞周期,计算S期细胞比率(SPF)和DNA指数(DI);