Methods: MIC was determined by microdilution method.BBF was established by plate culture method and defined by being stained with AgNO3.Viable bacteria were counted by serial dilution.

• 方法：采用平板法培养细菌生物被膜，微量稀释法测定抗菌药物的最低抑菌浓度(MIC)，银染法快速鉴定BBF，连续稀释法进行活菌计数。